The response of blood plasma metabolite concentrations of anestrus lactating buffalo to CIDR-eCG with or without melatonin treatment

The objective of this study was to compare the efficiency CIDR-eCG protocol with or without melatonin treatment on blood plasma metabolites in anestrus Murrah buffaloes during out-of-breeding season. Twelve lactating were allotted control and treated groups each six animals. Treated implanted (18 mg / 50 kg body weight) for 45 days, then animals both received CIDR 9 days. All 500 IU eCG intramuscularly one day before removal 10 µg GnRH AI. subjected estrus detection daily as per farm routine. At weekly intervals, samples collected determine concentration over 84 days’ period between June September. exhibited after removal. preceded increased total protein, albumin, cholesterol HDL-cholesterol (P<0.05) comparison only control. Buffaloes plus (P<0.01) glucose days 28, 53 55 reduction HDL AST at time pregnancy diagnosis, while phosphorus highest 84. In conclusion, implantation improved reproductive performance altered metabolite composition season compared only.